Fig 4.TIF (4.43 MB)
Evaluation of CRISPR/Cas9 SAMs directed to Prnp.
figure
posted on 2016-04-29, 08:27 authored by Lech Kaczmarczyk, Ylva Mende, Branko Zevnik, Walker S. Jackson(A) Relative positions of SAM sgRNA spacers with respect to the Prnp promoter region. Binding sites for AP1 and AP2 transcription factors were highlighted. (B) Schematic of the experimental workflow for SAM sgRNA evaluation. (C) Preliminary screening of SAM guides; sqRT PCR histogram shows increases in PrP protein levels for each individual SAM sgRNA tested, normalized to untransfected control levels (set to 1); assays #1 and #2 refer to independent primer pairs directed (respectively) to 5’- and 3’-proximal region of PrP mRNA.
History
Usage metrics
Categories
Keywords
neurodegenerative diseasesCC 9 systemPrP expressionSAMmouse modelsunderstanding PrP functionsprotein conformersresearch programcell culture systemsyears genome engineering technologiesPrion Protein Gene Sequencefood animalsPrnp locusgene-targeting techniquesCRISPRmouse Prnp locusprion diseasesactivation mediatorsTALENCC 9-basedExpression Levelsprion protein
Licence
Exports
RefWorks
BibTeX
Ref. manager
Endnote
DataCite
NLM
DC