Public Library of Science
pone.0260645.g003.tif (2.42 MB)

Embryo culture in the thermal bottle.

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posted on 2021-12-23, 18:27 authored by Yasuyuki Kikuchi, Sayaka Wakayama, Daiyu Ito, Masatoshi Ooga, Teruhiko Wakayama

(a) The thermal bottle used in this study is commercially available (left). Microtubes with OptC medium and embryos were put in the bottle containing 38.5°C water (right). (b) Water temperature in the thermal bottle was measured for 48 h. (c) Embryos at the time of collection from the thermal bottle (upper) and embryos cultured for 3 to 4 days in a CO2 incubator after collection (lower). (d) The birth rates of offspring derived from embryos cultured in the thermal bottle for 1 or 2 days.