Embryo culture in the thermal bottle.

(a) The thermal bottle used in this study is commercially available (left). Microtubes with OptC medium and embryos were put in the bottle containing 38.5°C water (right). (b) Water temperature in the thermal bottle was measured for 48 h. (c) Embryos at the time of collection from the thermal bottle (upper) and embryos cultured for 3 to 4 days in a CO₂ incubator after collection (lower). (d) The birth rates of offspring derived from embryos cultured in the thermal bottle for 1 or 2 days.