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Electrochemical detection of wAlbB LAMP products (L) and PCR products (P).

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posted on 13.05.2022, 17:43 by Parinda Thayanukul, Benchaporn Lertanantawong, Worachart Sirawaraporn, Surat Charasmongkolcharoen, Thanyarat Chaibun, Rattanalak Jittungdee, Pattamaporn Kittayapong

PCR reactions were performed for the general wsp gene (81F/691R), wAlbA gene (328F/691R), and wAlbB gene (183F/691R). Different mosquito species were included as follows: wild-type Aedes aegypti (AegW, Aae-JJ), wAlbB trans-infected Thai Ae. aegypti (AegB, wAlbB-TH), and Aedes albopictus (Alb, Aal-CH). Samples giving peak currents above 30 nA (approximately four-times blank signal) were considered positive. The bar charts represent the mean of 5 replications of DPV measurement and standard deviation was shown. Different patterns indicate either PCR or LAMP reaction products. Colors indicate mosquito colonies.

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