Efficiency of siRna transfection and silencing.
(A) H295R cells transfected with a fluorescent siRna indicator. (B) CYP11B1 gene expression in CYP11B1 siRna cells and non-targeting siRna cells. Data obtained from two independent experiments with two replicates for each experiment. (C) Cortisol levels in CYP11B1 siRna cells and non-targeting siRna control cells. Four independent experiments were used to determine each data point.