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Efficiency of siRna transfection and silencing.

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posted on 2018-05-07, 17:34 authored by Antonina Germano, Laura Saba, Silvia De Francia, Ida Rapa, Paola Perotti, Alfredo Berruti, Marco Volante, Massimo Terzolo

(A) H295R cells transfected with a fluorescent siRna indicator. (B) CYP11B1 gene expression in CYP11B1 siRna cells and non-targeting siRna cells. Data obtained from two independent experiments with two replicates for each experiment. (C) Cortisol levels in CYP11B1 siRna cells and non-targeting siRna control cells. Four independent experiments were used to determine each data point.

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    H 295R ACC cellsadrenocortical carcinoma cells MitotaneCYP 11B catalyzesmitotane actionCYP 11Bmetabolisminfluence mitotane-mediated cytotoxic effect

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