Effects of DD concentration on cell growth and viability.

(A) Cell densities were measured with a hemocytometer. Error bars represent standard error. Significance calculated using student’s t-test, P< 0.05 for 25 µM, 50 µM and 75 µM DD with respect to DMSO solvent control and P> 0.05 for 10µM DD with respect to DMSO solvent control; n = 3. Representative data from three independent experiments are shown. (B) Measurement of viability in cells treated with DMSO and DD dose for the times indicated (hr). Cell accumulation of Sytox Green fluorescent dye was measured by flow cytometry. MFI is Median Fluorescence Intensity. Error bars represent standard error. Changes with 50 µM DD were found significant with respect to DMSO solvent control, student’s T-test, * p<0.05; n = 3.