γ-H2AX in the VTA of brain sections from k18hACE2 mice uninfected or infected with 5 × 104 PFU 6 days after administration. Bar is 100 μm. Western blot analysis of (B) γ-H2AX tissue lysates of the VTA from the brains of k18hACE2 mice uninfected or infected with 5×104 PFU 7 days after administration. The intensity of the bands corresponding to γ-H2AX was normalized to that of β-Actin. The data are representative of 6 mice per group, *P < 0.05, **P < 0.01.