ppat.1008473.s005.tif (10.82 MB)

Effect of RHIM mutation on the size of oligomers formed between ORF20 and ZBP1.

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posted on 10.07.2020 by Megan Steain, Max O. D. G. Baker, Chi L. L. Pham, Nirukshan Shanmugam, Yann Gambin, Emma Sierecki, Brian P. McSharry, Selmir Avdic, Barry Slobedman, Margaret Sunde, Allison Abendroth

(A) Photon count histogram of fluorescence intensity detected in mCherry emission channel reflects oligomer particle size distribution over a 3 min period for ORF201-114-mCherry alone or in combination with YPet-ZBP1170-355. (B) Photon count histogram of fluorescence intensity detected in mCherry emission channel reflects oligomer particle size distribution over a 3 min period for ORF201-114mut-mCherry alone or in combination with YPet-ZBP1170-355. (C) Fluorescence correlation analysis reveals distribution of particles of different sizes, reflected by correlation coefficient (tau).

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