Ectopic EWSR1-FLI1 interacts with specific DNA and protein-binding sites.
A. EWSR1-FLI1 immunoprecipitation using anti-FLAG antibody in parental HeLa3G cells (control) and two different clones (HeLa Tet-On EF #3.10 and #3.15) in the presence or absence of doxycycline. B. Binding of EWSR1-FLI1 to the CAV1 enhancer and the promoters of the CAV1, EZH2 and AURKB genes. qPCR analysis of EWSR1-FLI1 ChIP signals for HeLa Tet-On EF #3.15 clone. C. Co-immunoprecipitation of EWSR1-FLI1 3×FLAG C-terminal and LSD1 protein in HeLa Tet-On EF #3.15 clone.