Fig 7.TIF (885.89 kB)
EGFR signalling regulates clonogenic survival of HNSCC cells derived from forced suspension cultures.
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posted on 2016-09-19, 17:35 authored by Diana Braunholz, Mohammad Saki, Franziska Niehr, Merve Öztürk, Berta Borràs Puértolas, Robert Konschak, Volker Budach, Ingeborg TinhoferCells were cultured as monolayer (M) or in forced suspension (FS) in the absence or presence of EGF, AREG or CTX. 72h later, cells were harvested, disaggregated to single cells and subjected to clonogenic survival analysis. Bars represent the surviving fractions (SF) ± SD from three independent experiments. Significant changes compared to control are marked with an asterisk.
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form multi-cellular clustersanti-adhesive polymer poly -2-hydroxyethylmethacrylateHNSCC cellsspheroid formationCTX-sensitive cell lines-9P-9Repidermal growth factor receptorspheroid culturesbasal EGFR expression levelsanti-metastatic treatment approachcoating culture dishesHNSCC cell linesHNSCC cell lines FaDuCTC cluster formationUT-SCCanchorage-independent cell survivalcluster formation abilityAnchorage Independent SurvivalMAPKNeck Cancer Cells
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