Double reciprocals and secondary plots of 3-MP adding PEP and GDP as variable substrates.
figure
posted on 2016-07-08, 16:40 authored by Jorge Hidalgo, Pedro Latorre, José Alberto Carrodeguas, Adrián Velázquez-Campoy, Javier Sancho, Pascual López-Buesa(A-C) Double reciprocal, slopes and intercepts of 139Met varying PEP. (D-F) Double reciprocal, slopes and intercepts of 139Leu varying PEP. (G) Double reciprocal of 139Met varying GDP. (H) Double reciprocal of 139Leu varying GDP. 139Met and 139Leu were assayed as described in Materials and Methods with increasing concentration of substrates (50–600 μM for PEP, 10–100 μM for GDP) and 3-MP concentration between 10–100 μM.
