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Distribution of injected splenocyte-derived myeloid cells in healed mouse skin.

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posted on 23.06.2022, 20:05 authored by Yunyuan Li, Hatem Alnojeidi, Ruhangiz T. Kilani, Aziz Ghahary

(A) Schematic figure of Dil-labeled or GFP-expressing mouse myeloid cell lineage tracing experiments. Splenocytes were isolated from spleens of Balb/C or C57BL/6-Tg (UBC-GFP) mice and cultured in a medium containing 5 ng/ml of M-CSF to generate myeloid cells. One million labeled cells were dermally injected into the healthy skin at one spot where was one centimeter far from the edge of wounds at the same time of skin injury. (B) Dil-labeled cells in wounded skin of mice received nothing (Left) or one million Dil-labeled splenocyte-derived myeloid cells (Right). (C) GFP-positive cells in wounded skin of mice received nothing (Left) or one million GFP-expressing splenocyte-derived myeloid cells (Right). Skins were stained with GFP antibody (green). DAPI (blue) was used as a nuclear counterstain. Scale bars in all images were 100 μm.

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