Disease relevant human cytokine levels in hIL-2 NOG mice compared to C.B-17 scid and NOG mice.

Human IL-17A, IL-22, IFN-γ and TNF-α protein levels were analyzed in graft lysates (a, c, e, g) and serum (b, d, f, h) on day 15 and 43 by the MSD platform. Keratomes from six psoriasis vulgaris patients were included. The number of mice in each group was on day 15: C.B-17 scid n = 8; NOG n = 7; hIL2-NOG n = 6 and on day 43: C.B-17 scid n = 20; NOG n = 20; hIL2-NOG n = 11. Lysates were generated from biopsies obtained from keratomes at arrival (Three biopsies per keratome, n = 18) and grafts 15 and 43 days after surgery. Serum was isolated from blood obtained by cardiac puncture on day 15 and 43. Human IL-17A (a), IL-22 (c), IFN-γ (e) and TNF-α (g) protein levels in keratomes pre-engraftment and graft lysates from C.B-17 scid, NOG and hIL-2 NOG mice. Human IL-17A (b), IL-22 (d), IFN-γ (f) and TNF-α (h) protein levels in serum from NOG and hIL-2 NOG mice. LLOD is lower limit of detection and HLOD is higher limit of detection.