Dilution linearity and reproducibility of ddPCR results.

(A) ddPCR analysis was performed on a serial dilution of dystrophin cDNA templates representative for the Δ48–50, with or without exon 51, using Taqman assays with a probe detecting the exon 51–52 junction (DMD ex51-52.2) or exon 47–52 junction (DMD ex47-52) respectively. Concentrations ranged from >5,000 copies/μl down to <1 copy/μl. Dilution linearity is indicated by the coefficient of determination (R²). (B) ddPCR analysis of 8 technical replicates at different template copy numbers (from 1 to 1,000 copies/μl) using the DMD ex51-52.2 and DMD ex47-52 Taqman assays. Merged data of 8 replicates is indicated by solid markers. Data is represented as mean with 95% CI.