Differential abundance analysis.

(A) Input selection of Wilcoxon Rank Sum test; (B) Grouped box plot, x-axis represents taxa and y-axis represents log10(relative frequency); (C) An individual box plot for each taxon, x-axis represents the condition and y-axis represents relative frequency; (D) Volcano plot, x-axis represents log10(mean relative abundance) and y-axis represents Log2FC; (E) Heatmap for significantly identified taxa; (F) Summary table for the Wilcoxon Rank Sum test. Similar input is needed for the remaining pairwise methods such as, metagenomeSeq, DESeq2, Limma-Voom and edgeR and multiple group comparison Kruskal-Wallis test and ANOVA.

(TIF)