Diagrams illustrating the mapping of chimeric reads and quality control of lhCLIP libraries.

(A) The mapping pipeline for lhCLIP data. (B) The relationship of intra- and intermolecular chimeric reads to reference transcripts. (C) The lhCLIP libraries were quantified using a 2100 Bioanalyzer. (D) Western blot detected the immunoprecipitated hnRNPK. (E) Analysis of the hnRNPK monomer and dimer captured in IhCLIP by blue native gel electrophoresis. The gel image illustrates the Coomassie Blue G-250 stained complex of the hnRNPK monomer along with its antibody. It also displays a native protein mark at 300 KDa, alongside a visible denatured pre-stained protein marker ladder. (F) Analysis of IhCLIP input sample by blue native gel electrophoresis and blotting with anti-hnRNPK antibody. In the left panel, the bands developed through ECL are displayed, while the right panel exhibits a denatured protein marker ladder captured under white light. Notably, the hnRNPK monomer band is evident around 60 KDa. (G) Correlation analysis of the non-chimeric reads between the replicates of lhCLIP.

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