Dephosphorylation of pS936-TRP is delayed in rdgC^ΔSM and rdgC^ΔL flies but is accelerated in rdgC^ΔS flies.

A, Flies were light or dark adapted for 1 h before heads were subjected to Western blot analyses using α-pS936, α-TRP, and α-RDGC antibodies. B, Flies were dark adapted over night and then illuminated for various durations before heads were subjected to Western blot analyses. C, Signals obtained with the α-pS936-TRP antibody were normalized by those obtained with the α-TRP antibody and plotted as % of the values obtained at t = 0 s. N = 4–7, error bars show SEM. Asterisks indicate significance levels of differences between wild type and rdgC^ΔS for the respective time points as calculated by Student’s t-test. *, p ≤ 0.05; **, p ≤ 0.01; ***, p ≤ 0.001. D, Scatter plot showing relative S936 phosphorylation after 90 s of illumination as % of t = 0 s. Error bars show SD. The significance was calculated using ANOVA with Dunnett’s multiple comparison test. *, p ≤ 0.05; ** p ≤ 0.01.