Dendritic complexity of MCs that misexpressed dnMib1 was not altered.

(A) Illustration of EP of CAG-rtTA and TRE-EYFP without (control) or with TRE-dnMib1-EYFP (dnMib1) at E11.5, induction by Dox at E16.5 and analysis at E18.5. (B–F) Representative images (B), dendritic reconstructions (C) and dendrite analyses of MCs that were positive for EYFP (green) (D–F). MCs were labeled with DiI and analyzed as in Fig 1: control, n = 18 from 6 mice; dnMib1, n = 16 from 6 mice. The phenotypes were indistinguishable among embryos transfected with the same genes. Scale bar: 20 μm. There were no significant differences in the number of branch points, total dendrite length or length of the longest dendrite of MCs between control and dnMib1 (P ranged from 0.905 to 0.972).