Delayed differentiation of medial ventricular wall ependymal cells in Jhy^lacZ/lacZ mice.

(A, B) IF detection of S-100β in P5 coronal sections from Jhy^+/+ (A) and Jhy^lacZ/lacZ (B) brains. The boxed region in each large image shows the region of the inset, and the white bracket denotes the width of the cell layers marked by S-100β expression. A) Jhy^+/+ medial wall cells display a flattened, single cell-layered differentiated ependyma, while B) Jhy^lacZ/lacZ medial wall cells retain a pseudostratified undifferentiated appearance. (C) Schematic of lateral ventricle showing regions designated as medial wall and lateral wall, dorsal and ventral. (D-G) H&E staining of sections of P5 Jhy^+/+ (D, E) and Jhy^lacZ/lacZ (F, G) lateral ventricle medial ependymal walls. Jhy^+/+ ependyma has a differentiated appearance in both dorsal and ventral regions (D, E), while Jhy^lacZ/lacZ remains undifferentiated dorsally, but is largely differentiated ventrally (F, G). MW, medial wall; LW, lateral wall; LV, lateral ventricle; D, dorsal; V, ventral; L, lateral. Scale bars: 50μm (A-B); 20μm (D-G).