DNA damage in Mcm5 mutants.
Mcm5 expression in NB5-6 Lineage. Nplp1 expression and quantification of Eya expressing cells in control and Mcm5 mutants. Dimm and pMad expression in Mcm7 mutants. Immunostaining for cH2aX, Eya and proFMRFa in Control (A) and Mcm5 mutants (B) at stage 18h AEL (scale bar 50 μm). Detail of immunostaining for cH2aX, Eya and proFMRFa in the apterous cluster cells of Control (C) and Mcm5 (D) mutants (scale bar 5 μm). (E) Expression of Mcm5 protein detected by immunostaining in the NB 5–6, identified by the reporter line Lbe(k)-GFP, and Deadpan immunostaining (Dpn). (F) Expression of Mcm5 protein detected by immunostaining in the Ap cluster cells, identified by Eya. (G) Quantification of Eya expressing cells within thoracic segments T2 and T3 in control and Mcm5 mutants (U-Mann-Whitney test; n≥3 CNS per genotype, n≥ 9 hemisegments per genotype, n.s = non-significant). (H-I) Immunostaining for proFMRFa, Nplp1 and Dimm in Control (H) and Mcm5 mutant (I), at stage 18h AEL (scale bar 5 μm). (J-K) Immunostaining for proFMRFa, Eya and pMad in Control (J) and Mcm7 mutant (K), at stage 18h AEL (scale bar 5 μm). Merge and individual antibody images are shown in each panel. Genotypes: (A, C, E, F, H, J) w1118. (B, D, I) Mcm5exc222/Mcm5exc222 (K) Mcm7 f03462/Mcm7 f03462. Mcm3smu (F) dpa1 (G) Mcm5exc222 (H) Mcm7 f03462.