DEK is not a component of the replisome.

(A-C) HeLa S3 cells were pulse-labelled with EdU for 2.5–30 min and biotin-azide was covalently attached via click chemistry. After cell lysis, EdU-biotin containing DNA fragments were precipitated using streptavidin-coupled magnetic beads. Bound proteins (capture) were identified by Western blot analysis. (A) Scheme of iPOND pulse experiment. (B) Representative Western blots of input and capture samples using antibodies specific for DEK, PCNA and histone H3. (C) Densitometric analysis. The fold change of captured protein is displayed relative to the value of the 30 min time point. Band intensities of capture samples were normalized to the respective input smaples. Shown are mean values from five independent experiments. One-sided error bars represent the S.D. 2way ANOVA with Bonferroni posttest: * p≤0.05, ** p≤0.01, *** p≤0.001. (D-F) HeLa S3 cells were pulse-labelled with EdU for 10 min, followed by a chase into thymidine containing medium for 0–30 min. iPOND was performed as in (A-C). (D) Scheme of iPOND chase experiment. (E) Representative Western blots of input and capture samples using antibodies specific for DEK, PCNA and histone H3. (F) Densitometric analysis. The fold change of captured protein is displayed relative to the value of the 0 min time point. Band intensities were normalized as in (C). Shown are mean values from five independent experiments, one-sided error bars represent the S.D. 2way ANOVA with Bonferroni posttest: * p≤0.05, ** p≤0.01, *** p≤0.001.