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Cytokine production by JE vaccine specific T cells by intracellular cytokine staining (ICS).

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posted on 2017-01-30, 17:32 authored by Lance Turtle, Filippo Tatullo, Tanushka Bali, Vasanthapuram Ravi, Mohammed Soni, Sajesh Chan, Savita Chib, Manjunatha M. Venkataswamy, Prachi Fadnis, Mansour Yaïch, Stefan Fernandez, Paul Klenerman, Vijaya Satchidanandam, Tom Solomon

(A) Magnitude of T cell cytokine responses to JEV SA14-14-2 detected by flow cytometry/ICS in five participants showing positive responses. Data are the percentage of cells in the parent (CD4+/CD8+) gate staining for the indicated cytokine, above unstimulated values. Filled circles = CD4+ responses, open circles = CD8+ responses. Bars = median value of CD4+ responses only. (B) SPICE analysis of CD4+ T cell responses detected by flow cytometry. Data are the proportion of the response comprised of IFNγ+/IL2+/TNFα+ triple positive cells (red pie slices), double positive cells (yellow slices) and single positive cells (blue slices). Arcs indicate the proportion of the response producing individual cytokines: IFNγ (purple arcs), IL2 (green arcs) and TNFα (light grey arcs). Left pie chart: CD4+ responses at weeks 1–4 (n = 4), right pie chart: participant VA001/1 at week 16. Participant VA001/1 made a triple positive response at week 16 after vaccination, hence this participant is presented separately.

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