Counts of Fin Rays and Spines.

The counted features of the fins are numbered in Panel A, (see key below) and the Left Pectoral Fin Rays (11) as well as the Lower (6) and Upper (5) Branched Caudal Rays are colored blue, signifying that these counts are significantly greater In E. newberryi than they are in E. kristinae. Bar graphs in B and C chart the number of individuals of E. newberryi in blue and E. kristinae in red that have a particular number of counted rays (x axis) for Pectoral Fin Rays (11) and Lower Branched Caudal Rays (6) respectively. Significance was determined using the non-parametric Wilcoxon Summed Rank Test (implemented in JMP), p<0.0001 in the three cases (well within our a priori criteria of p<0.001). Counts related to them and combined with them (Right Pectoral, 11-Left + Right Pectoral; 5-Upper Branched Caudal Rays, Total Branched Caudal Rays (5+6) were also significant at this level. Panel D presents an ANOVA analysis of means for sampling locality for, 6-Lower Branched Caudal Ray count for heuristic comparison. Localities are displayed from north to south with the E. newberryi/E. kristinae geographic split shown by the blue red vertical bar. As can be seen, E. kristinae by site are consistently lower in this variable. Total branched Caudal Ray Count (5+6 not shown) provides a similarly consistent result. Thus, these counts appear to have modest utility in species diagnosis. Key to localities: A-Smith River, B-Lake Earl, C-Humboldt Bay, D-Brush Creek, E-Estero Americano, F-Rodeo Lagoon, G-Arroyo de Corral, H-Morro Bay†, I-Santa Inez River, J-Jalama Creek, K-Agua Caliente, L-Winchester Canyon†, M-Ventura River, N-Malibu Lagoon†, O-Aliso Creek†, P-San Mateo Creek†, Q-San Onofre Creek, R-Las Pulgas/Las Flores Creek†, S-San Luis Rey River†, T-Buena Vista Lagoon† (most of these habitats are lagoonal in nature, † = currently extirpated, based on absence in recent surveys). Key to fin spine and ray counts: 1-Number of dorsal fin spines, DSPINES; 2-Number of second dorsal fin ray elements, DRAYS; 3-Upper procurrent caudal fin rays, UPRORAY; 4-Upper unbranched primary caudal rays, UUNBRC; 5-Upper branched primary caudal rays, UBRCAR; 6-Lower branched primary caudal rays, LBRCAR; 7-Lower unbranched primary caudal rays, LUNBRCR; 8-Lower procurrent caudal rays, LPRORAY; 9-Number of Anal fin ray elements, ARAYS; 10-Pelvic fin rays, PELELEM; 11-Left pectoral fin rays, LPECRAYS; Right pectoral fin rays, RPECRAYS (not shown); Number of pre-caudal or abdominal vertebrae, PRECAUD (not shown); Number of post-caudal vertebrae (including last half centrum as one), POSTCAUD; (not shown) Total vertebrae, TOTVERT, (not shown). Counts of Fin Rays and Spines are tabulated and averaged by locality in S3 Table.