pone.0262060.g005.tif (2.44 MB)
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Conversion of GFP-expressing myeloid cells to keratinocytes in re-epithelialized wounded skin.

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posted on 23.06.2022, 20:05 authored by Yunyuan Li, Hatem Alnojeidi, Ruhangiz T. Kilani, Aziz Ghahary

(A) Double immunofluorescent staining with antibodies of GFP (green) and K14 (red) in healed skin from wounds of C57BL/6 mice received one million GFP-expressing splenocyte-derived myeloid cells. (B) Double immunofluorescent staining with K14 (red) and GFP (green) antibodies in cultured skin cells. Skin cells were isolated from wound edge of mice received GFP-expressing myeloid cells and injury for 14 days. Cells were cultured for 48 hours before fixation and staining. DAPI (blue) was used as a nuclear counterstain. Scale bars in all images was 50 μm.