Conversion of GFP-expressing myeloid cells to keratinocytes in re-epithelialized wounded skin.
(A) Double immunofluorescent staining with antibodies of GFP (green) and K14 (red) in healed skin from wounds of C57BL/6 mice received one million GFP-expressing splenocyte-derived myeloid cells. (B) Double immunofluorescent staining with K14 (red) and GFP (green) antibodies in cultured skin cells. Skin cells were isolated from wound edge of mice received GFP-expressing myeloid cells and injury for 14 days. Cells were cultured for 48 hours before fixation and staining. DAPI (blue) was used as a nuclear counterstain. Scale bars in all images was 50 μm.