Conversion of CD45-positive hematopoietic cells to keratinocytes in re-epithelialization skin of mice.
(A) Double immunofluorescent staining with keratin-14 (K14) and CD45 antibodies in normal skin (Top panels) and wounded skin (Bottom panels) of Balb/C mice. Wounded skin was taken from mice with 8 mm punch biopsy of excisional wounds after wounding for 14 days. (B) Double immunofluorescent staining with K14 and CD45 antibodies in cultured skin cells isolated from wound edge of Balb/C mouse skin. Skin cells were isolated from the edge of wounded skin of mice by collagenase digestion. Cells were cultured for 48 hours before fixation and performing an immunofluorescent staining. DAPI (blue) was used as a nuclear counterstain. Scale bars in all images was 50 μm.