Comparison of phylum level classification of PCR amplicon and RT-SSU rRNA sequence reads derived from canine plaque samples.

BION-meta was used to classify and compare sequence reads obtained from 16S rRNA gene PCR amplicons and RT-SSU rRNA from the same canine plaque sample. In order to compare the accuracy of BION-meta, the 16S rRNA amplicon dataset was also classified using Qiime and RT-SSU rRNA using the RDP classifier. The RDP database was used as a reference dataset for sequence classification with all three classifiers (RDP classifier, BION-meta and Qiime). Sequence QC and classification statistics are provided in S1 Table.