pntd.0008176.g007.tif (1.65 MB)
Comparing the viability quantified with fluorescence assays and microscopy.
figure
posted on 2020-03-26, 17:44 authored by Laura Braun, Lucinda Hazell, Alexander J. Webb, Fiona Allan, Aidan M. Emery, Michael R. TempletonSamples containing 100 ± 4 cercariae with a viability of 30% ± 2% were stained with FDA-PI and FDA-H. Measurements were taken at 20 minutes. Three 50 μl aliquots of each sample were then examined under the microscope, and cercarial viability was recorded by counting moving (viable) and non-moving (non-viable) cercariae. Average quantified viabilities and SEMs are shown on the bar graph. The “actual” viability is the known proportion of live and dead cercariae in each well, chosen as 30%.