posted on 2016-03-08, 08:20authored byJeongsu Oh, Chi-Hwan Choi, Min-Kyu Park, Byung Kwon Kim, Kyuin Hwang, Sang-Heon Lee, Soon Gyu Hong, Arshan Nasir, Wan-Sup Cho, Kyung Mo Kim
The clustering accuracy of CLUSTOM, CLUSTOM-CLOUD, DOTUR-AL-PSA, ESPRIT-Tree, mothur-AL-PSA, mothur-AL-MSA, UCLUST and Swarm was performed based on 16S rRNA pyrosequences of a mock community that was constructed by pooled DNA of 21 human-associated prokaryotic strains with even concentration (HMP-Mock-community). The precision and recall metrics as well as their F2 values were used to compare the clustering accuracy of the eight programs at the species (A) and genus (B) levels.