Clr4 and Set1 antagonistically regulate protein levels of Lsd1/2 proteins.

(A-D) Lsd1-FTP (A & C) and Lsd2-FTP (B & D) protein abundance were analyzed by SDS-PAGE and western blot in the loss of indicated COMPASS complex members (A-B) or CLRC complex members and the cul4 mutant (cul4-1) (C-D). Asterisks denote the expected full-length size of Lsd1-FTP (A) and Lsd2-FTP (B). (E) Examination of Lsd1-FTP and Lsd2-FTP protein levels via SDS-PAGE and western blotting in the wild-type, cul4-1, and ddb1Δ backgrounds. PAP was utilized to detect Lsd1/2 protein levels. Mlo3 levels were employed as the loading control.