Chromatogram from Sanger sequencing of a representative homozygous mutant clone and an isogenic control cell line.

The reference sequence if given at the top (location hg38 47337533 to 47337545). The site of the blocking mutation (to prevent re-cutting of the Cas9 nuclease) and the single nucleotide polymorphism (SNP) that results in the R820W mutation are highlighted. The isogenic control clone has the blocking mutation and is wild-type at the SNP location.