Characterisation of T. brucei 2T1 cell lines expressing N- or C-terminal tagged TbAQP2.

A) Proliferation was estimated over a period of four days in vitro in the presence or absence of tetracycline for ^3xHAAQP2 (middle panel) or AQP2^3xHA (right panel) cell lines. T. brucei 2T1 wild type aqp-null cell lines (left panel) were included as the parental strain. B) Dose-response curves for pentamidine from T. brucei 2T1 wild type of aqp-null cell lines (left panel), ^3xHAAQP2 (middle panel), and AQP2^3xHA (right panel). C) Dose-response curves for SHAM (left panels) or SHAM plus 5 mM glycerol (right panels), with the same lines as in panels A and B.

(AI)