Cellular localization of mCherry to the cytosol, cell membrane, and cell wall.
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posted on 2025-04-16, 17:34 authored by Kalisa Kang, Évellin do Espirito Santo, Crisandra Jade Diaz, Aaron Oliver, Lisa Saxton, Lauren May, Stephen Mayfield, João Vitor Dutra MolinoFluorescent microscopy demonstrated mCherry expression in the B) cytosol, C) secretory pathway, D) cell membrane, and E) cell wall. Scale bars indicate 5 μm. F, G, H) The recrystallized cell wall components of transgenic C. incerta pJPW2mCherry showed mCherry fluorescence, absent on the wild type. The recombinant strains were deposited at Chlamydomonas collection (Ci_pAH04mCherry CC-6206; Ci_pJP32mCherry CC-6207; Ci_pJPM1 CC-6208; Ci_pJPW2 CC-6209).
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polyester polyurethane suspensionimpranil ® dlngenetically close relativefluorescent protein mcherryenhance phenotypic diversitydegrading enzyme phl7additional reference pointrecombinant protein productionrecombinant protein genesclose genetic relationshipaugment genetic variationtarget protein expressionimmunoblot intensity compareddegrading enzyme xylanaserecombinant protein expressionvarious cellular compartmentsgenerate genetic fusionproduced approximately 3potential new candidatechlamydomonas reinhardtii chlamydomonas incerta model green algaxylanase expressionreinhardtii incerta cellular fusionrecombinant offspringgenetic hybridsgreen algaecompared directlycellular transformationvitro degradationstudy investigatesspecies offerssecretory pathwayrobust platformpowerful toolphotosynthetic organismsindustrial applicabilityfold increasefindings emphasizeenzymatic activitiescytoplasmic membranecould exchangecell wallbiotechnological applicationsalso providesalso exploredalso examined
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