Cell mortality rate of bovine trophoblasts infected with Listeria spp.

(A) Cell mortality was analyzed by measuring the LDH released in the supernatant of CAM explants infected with L. monocytogenes or non infected controls at 1 and 8 hpi. (B) Cell mortality was analyzed by measuring the LDH released in the supernatant of explants uninfected or infected with L. monocytogenes, L. innocua, or L ivanovii at 1 and 8 hpi. The value of 100% cell mortality was calculated as optical density measured for LDH of total trophoblastic cells from explants lysed with mix substrate Cytotox kit Non-Radioactive Cytotoxicity 96 Assay. Results are presented as median and range of the data of five independent experiments performed in triplicate (Mann Whitney, * p <0.05).