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Cell counts from immunofluorescence stained colonic tissue sections.

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posted on 2017-09-18, 17:31 authored by Troy Perry, Michael Laffin, Richard N. Fedorak, Aducio Thiesen, Bryan Dicken, Karen L. Madsen

(A) Cells labelled with CD11c and CD11b. In controls, DSS treatment decreased numbers of CD11c+ cells and increased numbers of CD11b+ cells. At day 16, ICR mice had decreased CD11c+ cells and increased CD11b+ cells. DSS treatment in ICR mice eliminated the rise in CD11b+ cells. At day 31, CD11c+ cells remained decreased in the ICR and ICR-D groups, but CD11b+ cells had increased in both groups. Data is shown as Mean ± SEM. Counts were performed on 6 representative photomicrographs from n = 3/group. * p ≤ 0.05 relative to Ct. (B) Cells labelled with CD11c and CD103. ICR and ICR-D mice had increased numbers of CD11c+CD103+ cells at day 16 and at day 31. Data is shown as Mean ± SEM. Counts were performed on 6 representative photomicrographs from n = 3/group. * p ≤ 0.05 relative to Ct and Ct-D. (C) Cells labelled with F4/80 and NOS2/F4/80. At day 16 and 31, ICR and ICR-D mice had increased levels of F4/80+ cells. Data is shown as Mean ± SEM. Counts were performed on 6 representative photomicrographs from n = 3/group. * p ≤ 0.05 relative to Ct and Ct-D; # p ≤ 0.05 with Ct; +p<0.05 compared with Day 16 ICR and ICR-D.

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