CaCl2 treatment causes a significant reduction in Pol I occupancy, but the addition of MNase only results in mild changes to occupancy in the spacer regions.
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posted on 2025-02-13, 18:37 authored by Abigail K. Huffines, David A. SchneiderCaCl2 treatment causes a significant reduction in Pol I occupancy, but the addition of MNase only results in mild changes to occupancy in the spacer regions.
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untreated control samplesgeneration sequencing techniquessaccharomyces cerevisiae nascent transcript capturenascent rrna compareddiv >< pseq ), allowsprocess called transcriptionbacterial rna polymerasedefining polymerase occupancyii recently optimized polpol ii netnascent rnarna polymerasevivo polymerase stallingspecific polsynthesize rnarna polymerasesunique characteristicstaken togetherstudy transcriptionsignificant reductionseveral nextseq causedoriginally developedomitted nucleasesobservations reinforcenucleotide resolutionnucleotide additionmetal treatmentmetal cofactorsdna templatesdna templatedivalent metalsdifferential impactcommonly used>, however&# 177
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