CaCl₂ and MnCl₂ treatment significantly reduced Pol I occupancy on the rDNA template.

(A) Top panel: the mean Pol I occupancy was plotted for both the untreated and CaCl₂ treated samples. At each position within the rDNA template, a t-test was performed, and a p-value was calculated. The p-value was adjusted via the p.adjust function in RStudio (method = “BH”). If the p-value < 0.05, that was deemed a significant difference between the two treatment groups and was indicated with either a green (increased occupancy) or black (decreased occupancy) line below the histogram for the CaCl₂ treated samples with respect to the untreated samples. Bottom panel: the moving average across 200 nucleotides was plotted for both the untreated and CaCl₂ treated samples. (B) Plots were generated as described in (A) for the untreated samples vs. MnCl₂ treated samples.