CI vs. Fa plots (combination index vs. fraction affected) for different treatment fashions using CompuSyn software.

MDA-MB-231 (a), MCF 7 (b), and MDA-MB-468(c) cells were treated with a mixture of enzalutamide and ribociclib (0.5, 2, 5, 10, 15, 20, 25, 35, 45, and 60 μM of enzalutamide in combination with 5 and 25 μM of ribociclib) for 48 hr. Cell viability was investigated by MTT assay. CI vs. Fa plot for cell viability data. CI values less than 1 are recognized as having a synergistic effect. The mean of three independent experiments was used to calculate the results.