CD45-positive hematopoietic cells in skin wounds of mice.

(A) Immunofluorescent staining in normal and wounded skin of mice with CD45 antibody. Balb/C mice with eight-millimeter punch biopsy excisional wounds for 14 days were euthanized and wounded skin and normal dorsal skin were harvested and fixed. Skin sections were prepared for immunofluorescent staining with CD45 antibody (green). DAPI (blue) was used as a nuclear counterstain. Scale bars in all images was 50 μm. A representative of wounded skin of 16 wounds from 8 mice was shown. (B) The statistical analysis of CD45 positive cell percentage in skin sections is depicted. Ten HPF of five normal and five wounded skin were counted (n = 5).