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Bradyzoite differentiation to tachyzoite in human intestinal microphysiological systems.

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posted on 2025-02-04, 18:40 authored by Carlos J. Ramírez-Flores, Nicole D. Hryckowian, Andrew N. Gale, Kehinde Adebayo Babatunde, Marcos Lares, David J. Beebe, Sheena C. Kerr, Laura J. Knoll

(A) Fluorescent images showing replication of alive ME49 mCherry bradyzoites in the Caco-2 lumen. (i)(ii), and (iii) show immunofluorescent images of the parasite conversion of ME49 mCherry bradyzoites to tachyzoites, with markers for tachyzoites (SAG1, red) and bradyzoites (BAG1, green) at 3 days post-infection. (B) Fluorescent images of alive EGS LDH2p-GFP/SAG1p-mCherry parasites undergoing differentiation in MPS, with bradyzoites shown in green and tachyzoites in red. Punctuated lines represent the edges of the lumen and its boundary with the matrix. Lumens in (A) and (B) were infected with a total of 3 × 104 bradyzoites ME49 mCherry or EGS LDH2p-GFP/SAG1p-mCherry parasites. Plots represent the quantification of mCherry positive PVs (expressing SAG1) from day 2- to 5-post infection as bradyzoites. Plots are graphed as the average of positive PVs per lumen. A total of seven plates were analyzed, each one with at least three infected lumens.

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