Fig 9.TIF (1.04 MB)
Both immuno- and standard subunits of the proteasome are constitutively expressed in leukocytes and liver.
figure
posted on 2016-04-21, 06:00 authored by Felicia Kirschner, Katrin Reppe, Nadine Andresen, Martin Witzenrath, Frédéric Ebstein, Peter-Michael Kloetzel(A) Analysis of proteasome catalytic β-subunits expression on protein level in liver, 48 h after transnasal application of 5×106 CFU PN36/mouse, performed by immuno-blot. (B) Analysis of proteasome catalytic β-subunits expression on protein level in BMM performed by immuno-blot (each group with n = 3). (C) 2D gel electrophoresis of purified 20S proteasomes, isolated from WT BMM. Proteasome subunits were visualized by coomassie stain. (D) Determination of β-subunits, incorporated in BMM 20S, detected by immuno-blot.
