Biomarkers of oxidative stress in liver tissue of rats stressed by restraint and cold.

Total antioxidant capacity by FRAP method (A). Lipid Peroxidation by TBARS method (B). No stress (NS), No Stress supplemented with Royal Jelly (NSRJ), Stress (S) and Stress supplemented with Royal Jelly (SRJ). Values are expressed as mean±SEM, * p < 0.05 vs. NS, # p < 0.05 vs. S (One-way ANOVA followed by Tukey test). Pearson correlation of FRAP and TBARS (panels C and D) and means values of corticosterone levels. Outliers were detected by performing Grubb’s test using an online GraphPad outlier calculator (http://graphpad.com/quickcalcs/Grubbs1.cfm).