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Basic temporal response types.

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posted on 2014-01-20, 03:26 authored by Alberto Capurro, Fabiano Baroni, Linda S. Kuebler, Zsolt Kárpáti, Teun Dekker, Hong Lei, Bill S. Hansson, Timothy C. Pearce, Shannon B. Olsson

(A) A biphasic neuron from M. sexta stimulated with a mixture (upper panel) and a single component at the mixture concentration (lower panel). (B) An excited neuron stimulated at low (upper panel) and high (lower panel) concentrations of a compound. (C) An Inhibited neuron stimulated with low (upper panel) and high (lower panel) concentrations of a compound. Stimulus elapse shown in grey. The panels on the right show confocal micrographs of the three respective female M. sexta AL neurons, each extracting a single optical orthogonal slice (soma with asterisks, left images). Neurobiotin-injected cells were stained with Alexa-conjugated Streptavidin. Pictures were obtained by confocal microscopy of three separate whole mount brain preparations using a 10×, 0.45-NA objective lens (C-Apochromat, Zeiss). Optical sections (1024×1024 pixel) were taken at intervals of 0.8 µm. A and B display LNs, while C shows a multiglomerular PN; scale bar: 50 micrometers.

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