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Assessment of antipodal cells’ cDNA quality.
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posted on 2016-11-22, 17:40 authored by Xiaobo Yu, An Luo, Meng-xiang SunA. The gel electrophoresis analysis of antipodal cell cDNA. B. Alignment 2100 Bioanalyzer analysis of antipodal cell cDNA. Ladders from the bottom to the top are 35 (green), 50, 100, 150, 200, 300, 400, 500, 600,700, 1000, 2000, 3000 and 7000bp (purple) respectively. C. The verification of antipodal cell-specific gene expression by semiquantitative RT-PCR. D. The central cell specific maker FWA and DD45 were used as control to test possible contamination.
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antipodal cell isolationantipodal cell specificationcell type specificationArabidopsis thalianaPCRantipodal cell cDNAcell fate determinationcell type-specific marker genesembryo sac component cellsantipodal cellscell type-specific gene expression patternsCell Type-Specific GenesEfficient Antipodal Cell Isolation Methodtranscriptome analysis
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