Archazolid does not upregulate the expression of cell adhesion molecules in HUVECs.

(A) Confluent HUVECs were treated with 1 nM archazolid (arch) or DMSO (co) for 12 h. The expression of ICAM-1, VCAM-1, E-selectin and N-cadherin was analyzed on the mRNA level by qPCR experiments. (B) Confluent HUVECs were treated with 1 nM archazolid (arch) or DMSO (co) for 24 h. The surface expression of ICAM-1, VCAM-1, E-selectin and N-cadherin was analyzed by flow cytometry. (A, B) Data are expressed as mean ± SEM (n = 3). *p ≤ 0.05 versus DMSO control. TNFα (TNF) served as positive control to induce both the mRNA (1 ng/ml TNF) and the cell surface expression (10 ng/ml TNF) of ICAM-1, VCAM-1, E-selectin and N-cadherin.