Archazolid alter the cell morphology and is functionally active in HUVECs.

Confluent HUVECs were treated with 1 nM archazolid (arch) or DMSO (co). (A) Microscopic images of fixed cells were taken. Scale bar represents 100 μm. (B) Fluorescence microscopic images of viable cells were taken. LysoTracker Red DND-99 was used to visualize the acidic compartments (lysosomes) and Hoechst 33342 (blue) staining was used to visualize nuclei. Scale bar represents 20 μm. (A, B) One representative image out of three independently performed experiments is shown.