Apoptosis and cell cycle analysis of DU-145 RB60U Cells.

(A, B) The DU-145 RB60U cell clone maintains the apoptosis evasion observed on the DU-145 RB60 cells after a 24-week drug deprivation with a slight increase of its apoptotic rate. (C, D) Following the same procedures as in naïve and DU-145 RB60 cells, the cell cycle of DU-145 RB60U cells was analyzed using PI and indicated a mild G2 arrest following incubation with 60 nM of Bortezomib for the first time after 24 weeks. The subsequent cell cycle inhibition does not result in apoptosis as has been shown by the rest experiments assessing apoptosis compared to the naïve DU-145 that within 48–72 h undergo apoptosis.

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