Angular distribution of the direction of maximum mean squared displacement (soft direction), θ_MSD,max, in the apical and the basal regions of HeLa cells without vs. with EHEC infection.

(A) Apical region of HeLa cells without EHEC infection (data deduced from 112 particles; (B) basal region of HeLa cells without EHEC infection (data deduced from 158 particles); (C) apical region of EHEC-infected HeLa cells (data deduced from 66 particles); (D) basal region of EHEC-infected HeLa cells (data deduced from 55 particles). Statistically significant differences (obtained from Watson's U₂ test for angular distributions) were found between apical and basal regions of EHEC-infected cells (**, p<0.01), and between basal regions of non-infected and EHEC-infected cells (*, p<0.05). Variance and kurtosis of the angular distributions of θ_MSD,max are given in Table 2.