Amino acid substitutions that model PCH1b in Rrp40 alter levels of RNA exosome subunits.

(A) Lysates prepared from heads of control Rrp40^wt or Rrp40 mutant flies were resolved by SDS-PAGE and analyzed by immunoblotting with antibodies to detect RNA exosome Cap Subunits, Rrp40/EXOSC3 and Rrp4/EXOSC2, and Core Subunit Mtr3/EXOSC6. Both LaminD and Stain Free, as a measure of total protein, serve as loading controls. (B) Results from (A) were quantitated for RNA exosome cap subunits levels (Rrp40 and Rrp4- bands for Mtr3 had too much background to yield reproducible results) and are presented as Relative Protein Levels with the value from the control Rrp40-WT flies set to 1.0. Asterisks (*) indicate results that show statistical significance at *p-value< 0.05. Results that show no statistical significance when compared are indicated by NS.