Agrobacterium strains (EHA105, GV3101 and LBA4404) harboring binary vector pMDC45.
figure
posted on 2016-06-28, 18:40 authored by Ramachandran Srinivasan, Kodiveri Muthukalianan Gothandam(A) Schematic map of binary vector pMDC45. RB, Right border; LB, Left border; GFP, Green fluorescent protein gene. (B) Confirmation of Agrobacterium strains EHA105 (Lane 1 and 2), GV3101 (Lane 3 and 4) and LBA4404 (Lane 5 and 6) with pMDC45 by colony PCR, M—500 bp Marker in kb. The gel shows the corresponding 493bp fragments obtained with GFP-specific primers.
