pone.0230915.g005.tif (544.29 kB)
Agarose gel electrophoresis of PCR products using primer pair 1 and 13.
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posted on 2020-03-27, 20:05 authored by Jenq-Kuen Huang, Kadidia Samassekou, Hekmat B. Alhmadi, David R. VanDerway, Joshua D. Diaz, Jacob A. Seiver, Shawn W. McClenahan, Scott M. Holt, Lisa WenLane 1: genomic DNA from N. cholesterolicum NRRL5767 was the template. Lane 2: genomic DNA from clone 1-p-11 was the template. Lane 3: genomic DNA from the knockout mutant 1-3-17 was the template. Lane 4: genomic DNA from the knockout mutant 2-3-52 was the template. Lane 5: Hi-Lo DNA marker.
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Nocardia cholesterolicum NRRL 5767. Existencealcohol dehydrogenase geneknockout mutants offer improvementsoleate hydratase isozymesNocardia cholesterolicum NRRL 5767Nocardia cholesterolicum NRRL 5767DNAGolden Gate Assemblyalcohol dehydrogenase activityNocardia cholesterolicum NRRL 5767 strainalcohol dehydrogenase knockout mutants10- hydroxystearic acidoleic acid10- ketostearic acidCRISPRalcohol dehydrogenase
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