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Agarose gel electrophoresis images from three different dyes.

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posted on 2020-10-15, 17:37 authored by Eduardo Nogueira Cunha, Maria Fernanda Bezerra de Souza, Daniel Carlos Ferreira Lanza, João Paulo Matos Santos Lima

Ethidium bromide (A, B); GelRed (C, D); and SYBR Green (E, F). Photos obtained from the use of a conventional UV-transilluminator are on the left panel (A, C, and E). Images captured from the proposed device are on the right panel (B, D, and F). All photos underwent the same image treatment in the ImageJ software [21] so that there was no advantage for comparative analysis. Samples in the gel lanes are serial dilutions of the PCR final product (see Material and Methods). M–HighRanger 1 Kb DNA Ladder molecular marker (Norgen Biotek Corporation); DNA quantities: 1–228 ng; 2–114 ng; 3–57 ng; 4–28.5 ng; 5–14.28 ng; 6–7.14 ng; 7–3.54 ng; 8–1.8 ng).

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